In order to gain more insight into functional relevance of this atypical linkage of ubiquitin to the substrate protein via a thioester bond to a cysteine, we substituted this conserved amino acid by site directed mutagenesis to alanine or lysine.
Silencing of tumour-relevant molecules is becoming more and more relevant in analyzing the functional relevance of individual molecules.
In order to more fully understand the functional relevance of these patterns, we examined not only enrichment for biological annotations in the triplets themselves, but also the position within the triplet for any such enrichment.
Comparing the methylation and gene expression profiles of myometrium, placenta and umbilical cord blood of preterm and term deliveries could shed more light on the functional relevance of the methylation changes observed in the cord blood DNA.
However, numerous induced genes were found to be associated with more specific annotations of potential functional relevance (table 1 and supplementary data 3).
As a more cogent test of the functional relevance of each component, we next asked whether the ability of CBX7 to downregulate p16INK4a was dependent on either BMI1 or MEL18.
Our results encourage further and more focused studies on the functional relevance of particular adipogenic candidate genes.
To obtain a more general view of the functional relevance of LUAT-associated genes, we analyzed recently published directional and PolyA-enriched RNA-seq data from 17 mouse tissues [ 30].
Although these chromosomes comprise as much as 12%% of the total genome and encode more than 700 genes, no functional relevance has been assigned to accessory chromosomes in this species [ 11, 12].
As it is always complicated to interpret null results, our data point more to a lack of functional relevance of the hippocampus in particular memory processes like proactive interference or total learning than to the functional relevance of the prefrontal cortex in these processes.
