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Indeed, this approach did bring us more functional enzymes.
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The work presented here springboards off of the work of Li et al. [ 4] and provides more functional characterization of the enzymes expressed.
Compared with other strategies, shotgun library was much more common and easier to operate in discover functional enzymes.
We used PCR mutagenesis, screened >1000 DHFR alleles that encoded functional enzymes and studied ≈100 that were more resistant than a naturally occurring resistant allele (N51I and S108N).
The functional active sites of these two enzymes were quantified using the single turnover substrate DNPFG, and consistent with the hydrolytic activity, ∼5 7% more functional active sites were quantified with Vela than with Imig (Table 1).
Concerning Fungi, the PAL cluster contains the few characterized fungal enzymes (Amanita, Rhodotorula, Aspergillus) and is thus likely that the other orthologues have also PAL activity, although more functional data is needed to verify this.
Elbaz takes a more functional view.
The restaurant is more functional than fancy.
Extremes of fashion are out, replaced by more functional styles.
It's just a matter of making it more functional".
"Many people think of clothes in a more functional way.
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