Neurosphere cultures cannot fully recapitulate the in vivo cortical microenvironment, but they do provide a more supportive tissue-like environment in which hNPCs are known to thrive relative to dissociated cultures [23].
However, most mouse models of colitis do not fully recapitulate the pathophysiology of human IBD and generally only cause colonic inflammation (Fig. 4), which is more similar to ulcerative colitis (reviewed in Wirtz and Neurath, 2007 and discussed further below).
Furthermore, the exogenous addition of soluble factors was not able to fully recapitulate this phenotype that was imparted by shear stress exposure.
Although murine models do not fully recapitulate human infection, they are commonly used to evaluate EHEC vaccines and the immune/protective responses elicited in the host.
However, in transgenic assays, the GCR was unable to fully recapitulate all aspects of the endogenous Hoxd expression patterns during distal limb development.
They were therefore unlikely to fully recapitulate the biology of primary in vivo tumor development.
Although cell lines cannot fully recapitulate all the biological aspects of tumors [11], the chromosomal alterations observed in cell lines are representative of their parent histology [17].
Thus, it is not surprising that the miR-130a transgenic embryos do not fully recapitulate the phenotype of the FOG-2−/− embryos.
However, use of 50% ascites fluid alone does not fully recapitulate the ovarian tumor environment, which also contains immunosuppressive cell types such as Tregs and MDSCs [1], [2].
However, nanoparticles in solutions with physiological salt concentration, pH and temperature cannot fully recapitulate different types of cells or immune system in biological system.
