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Interestingly, the 1ptel-1p31 and 7p22-p15 regions were also shown to be more frequently gained in the MFHs, whereas the 1q32-qtel region was shown to be more frequently gained in the leiomyosarcomas, consistent with our findings.
A set of 24 locus-specific FISH probes directed against DNA sequences localized in 20 different human chromosomes, specific for those chromosomal regions more frequently gained or deleted in sporadic CRC [4], [6], [8], [17], [18] were systematically used to validate the results obtained with the SNP arrays (Table 1).
EMSY (p = 0.004) and CPD (p = 0.001) copy number gain were also independent predictors of gender and these genes were more frequently gained in female breast cancer.
If for a certain SNP locus one allele is substantially more frequently gained than the other allele, one may hypothesize that the former allele is subject to a larger selective pressure to change copy number.
Such nested gene structures in eukaryotes are generally much more frequently gained than lost, suggesting that while intronic insertions may be relatively well-tolerated, deletions are more likely to be constrained due to their possible negative impact on both the nested and the host genes [ 26].
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Chr.19 and chr.22 were more frequently lost in lines whereas they were more often gained in tumors.
"We did events more frequently, and gained some real momentum with Footwork, in the final few years," says Mastro.
Interestingly, five kinases (PTK2B, PHKB, DCAMKL1, TEK, MAP2K4) were deleted at least five times more frequently than gained, suggesting these kinases may play a role in negatively regulating growth.
Duplicate genes become pseudogenes much more frequently than gain new functions (Lynch and Force 2000; Nei and Rooney 2005).
It is possible that this finding may be explained by user bias, in that these patients may have activated the healthcare system more frequently to gain a diagnosis of an otherwise silent condition.
In contrast, gain of 12p has been inconsistently reported in childhood MGCTs, with gain of 12p13-pter observed more frequently than gain of more proximal 12p (Oosterhuis and Looijenga, 2005).
Related(15)
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