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Δ5q, Δ13, Δ9, Δ5 and ▼1aA were significantly more frequently annotated as predominant in breast tumor samples than in blood-derived samples.

We found that age-methylated CpG sites were over-represented in the gene body compared to age-demethylated CpG sites (52.5% vs 34.9%, χ = 39.8, P < 0.0001), and age-demethylated CpG sites were more frequently annotated within 1,500 bp of the transcriptional start site (TSS) compared to age-methylated sites (22.4% vs 8.93%, χ = 41.3, P < 0.0001), Figure  3C.

Additionally, we found that likely inactivating AS events were, on average, more frequently annotated as predominant in breast tumor samples than in healthy breast samples (2.6 vs 1.0; P = 0.020), while the same effect was not true for other AS events (4.1 vs 4.0; P = 0.689).

Similarly, likely inactivating splicing events (PTC-NMDs, Non-Coding, Δ5 and Δ18) were more frequently annotated as predominant in tumor than in normal breast samples (P = 0.020), whereas there were no significant differences for other splicing events (No-Fs) frequency distribution between tumor and normal breast samples (P = 0.689).

Focusing on individual AS events, we found that seven (Δ5q, Δ13, Δ9, Δ5, ▼1aA, Δ10 and ▼4, all of them likely inactivating) were more frequently annotated as predominant in breast tumor samples than in blood-derived samples, albeit in the case of Δ10 and ▼4 the findings did not reach statistical significance (Table  1).

Similar(55)

They were once part of a rental library, whose users frequently annotated them with comments, letters and Post-its.

These 'ORFans' are frequently annotated as 'hypothetical protein' and may in cases be falsely predicted genes.

The bacterial homologs are frequently annotated as 'hemolysin III', suggesting the role of a cytotoxin.

By contrast, film readers using paper forms frequently annotate the forms to record additional information.

In spite of their lower frequency among unique sequences, contigs were annotated more frequently (59.9%) than singletons (40.1%).

Note that none of the 27 AS events listed is annotated more frequently as predominant in blood- derived sample.

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