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We also used the mapping panel from Voss and Smith (2005) to map additional loci and more finely map the position of met1.
The continued erosion of LD in this population over subsequent generations, combined with the availability of more dense SNP panels, creates a unique opportunity to more finely map the location of QTL that are in LD with a causal mutation.
Data from the SILs were used to more finely map scaffolds that had previously been assigned to a single cluster due to absence of F2 hybrids that are recombinant for these scaffolds (see Materials and Methods).
In order to more finely map the effect of these mutations, the -RAKR- motif within the 18 and 20aa versions of pGFP-F2A-CherryFP (encoding the least active F2As) was mutated to - AAK A-, pGFP-F2A20Fm-CherryFPm-CherryFpGFP-F2A18Fm-CherryFPerryFP and Table 1).
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A locus distance that is more finely mapped than in previous studies is conducive to cloning this QTL; qpl4-1 isimilarar to the locus interval mapped by (Zhuang et al.1997).1997
LOD in the population reaches 97.92 with a contribution rate of 44.3% as shown in Figure 4A; qph1-1 is located on the short arm of chromosome 1 within a 14.6 kb interval, just in the area of ph1.1 and compared with the more finely mapped region by (Marri et al.2005).2005
Putative divergent chromosome regions will be more finely mapped to get a better approximation of their size and gene content.
These larger-capacity RAs mean more finely detailed mapping.
Mutations can then be mapped more finely by classical genetic mapping via the sexual cycle (Todd et al. 2007b).
We mapped recognition more finely within residues 21 151 using a series of N-terminal truncation mutants (Fig. 2E).
The situation is now more finely balanced.
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