Unlike recently developed methods used to map 3'UTRs, such as 3P-Seq [ 6] and FANS/3'-end-seq [ 24], SPIA generates cDNA libraries that cover the entire transcript, allowing for more extensive downstream transcriptome analysis within the same experiment, such as coupling gene isoform mapping with the study of 3'UTR dynamics.
We are aware that RNA-Seq enables a more extensive profiling of the transcriptome by facilitating the discovery of fusion genes [ 20], detection and quantification of alternative splice forms, and characterization of expressed mutations and polymorphisms [ 14].
More extensive analyses of monocyte/macrophage transcriptomes, aimed at the identification of common and unique activation pathways, will likely reveal the relevance of these transcriptional changes to important physiological processes and to the pathogenesis of diseases in which monocytes/macrophages play a role.
Pairwise differences were even more extensive: 77% of the measured transcriptome exhibited a significant difference among at least two strains measured in a common copper environment, whereas 67% exhibited a response to copper across the reaction norm of at least one strain.
We find at doses producing similar toxicity, exposure to non-selective γ-radiation results in more extensive reprogramming of the S. cerevisiae transcriptome than exposure to each of the three enediynes.
On the other hand, only 7,099 of the 72,072 conreads from Z. filipendulae, corresponding to 9.8%, are represented by Heliconius conreads, which most likely reflects the more extensive sampling of the Z. filipendulae transcriptome.
As expected, striatal transcriptome differences were far more extensive between the independently derived progenitor strains than between the quasi-congenic strains and their background partner, B6By.
In contrast to previously reported results, we have found that the transcriptomes for both of these metalloregulators are more extensive than anticipated.
Whilst these studies represent extensive transcriptome profiling of embryonic gonads, deep RNA sequencing (RNA-seq) is considerably more sensitive [ 39].
