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We also found more extensive staining in positive cases (Ginestier et al, 2007).
Stronger or more extensive staining was scored as moderately/strongly positive (2+/3+).
In contrast, ESCC tissue showed stronger or more extensive staining than corresponding non-neoplastic esophageal mucosa (Fig. 2c).
In one tumour most areas had only a few positive cells, although there was one region with more extensive staining.
We then assessed a muscle sample from an older mutant mouse (131 days) and found a similar pattern but with more extensive staining (Fig. 6).
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Three of the four normal liver samples showed slightly more extensive nuclear staining involving <10% of hepatocytes.
Of the tumours, 87% showed more extensive nuclear p53-positive staining than normal epidermis (e.g., Figure 6D).
Biopsies of invasive cervical carcinomas also showed a good correlation between staining for the two hypoxia markers, CA9 staining being more extensive in almost all cases (Olive et al, 2001).
Not all nuclei showed continua, but we observed that those that did not have continua showed more intense foci, while those with smaller focal staining had more extensive continual filaments (compare Fig. 4B,C to 4D).
We did not observe nuclear β-catenin labeling, and β-catenin staining was more extensive in the cytoplasm of the cells with more severe lesions (Fig. 2A,B).
Only a small population of cells stained for lipid droplets, but those that were positively stained showed more extensive droplet accumulation compared to K+-treated cells.
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