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Although use of more extensive sequences in the studies may have better characterized the identified abnormalities, they may also have identified additional abnormalities, so that the true prevalence of such lesions may be underestimated.
Interestingly, however, the C-terminal sequences responsible for activation of CA9 or PHD3 were not congruent between the HIF- α isoforms, with more extensive sequences being required for the activation of CA9 by HIF-1 α sequences.
In comparison with other Western Hemisphere hantaviruses for which more extensive sequences were available (1,287 nt) 75.8%84.1%% nucleotide sequence identity and 85.3% 97.7% amino acid identity were observed, and the highest similarity was with members of the species Andes virus (Table 1).
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Alt-EJ operates with speed and fidelity markedly lower than c-NHEJ, causing thus with higher probability chromosome translocations, and generating more extensive sequence alterations at the junction.
A more confident analysis would require much more extensive sequencing data, or the use of microarrays.
The demonstration of more extensive sequence diversity in buffalo-derived parasites than in those maintained in cattle is consistent with the idea that a limited subset of the T. parva population has become adapted for maintenance by tick transmission in cattle, or that many parasite genotypes cannot be maintained in the cattle tick transmission cycle.
As expected, at the protein level the Tp2 antigen showed more extensive sequence diversity than Tp1 and both antigens displayed greater diversity in parasite isolates from buffalo or cattle grazing with buffalo compared to parasites from cattle in buffalo-free areas.
More extensive sequencing of JUQ prototype and JUQ-like sequences is needed to clarify this point.
More extensive sequence information would be invaluable to complete the picture of the melon genomic landscape.
To further clarify the phylogeny of the Orthoptera, more extensive sequencing of orthopteran mitochondrial genomes is required.
mRNA-Seq is a powerful tool to develop SNPs but has some limitations for detecting more extensive sequence variation.
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