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Alt-EJ operates with speed and fidelity markedly lower than c-NHEJ, causing thus with higher probability chromosome translocations, and generating more extensive sequence alterations at the junction.
The demonstration of more extensive sequence diversity in buffalo-derived parasites than in those maintained in cattle is consistent with the idea that a limited subset of the T. parva population has become adapted for maintenance by tick transmission in cattle, or that many parasite genotypes cannot be maintained in the cattle tick transmission cycle.
As expected, at the protein level the Tp2 antigen showed more extensive sequence diversity than Tp1 and both antigens displayed greater diversity in parasite isolates from buffalo or cattle grazing with buffalo compared to parasites from cattle in buffalo-free areas.
More extensive sequence information would be invaluable to complete the picture of the melon genomic landscape.
mRNA-Seq is a powerful tool to develop SNPs but has some limitations for detecting more extensive sequence variation.
More extensive sequence analysis of available ZIKV strains of various temporal, geographic, and passage histories may provide some insight into this issue.
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A more confident analysis would require much more extensive sequencing data, or the use of microarrays.
More extensive sequencing of JUQ prototype and JUQ-like sequences is needed to clarify this point.
To further clarify the phylogeny of the Orthoptera, more extensive sequencing of orthopteran mitochondrial genomes is required.
Recent publications showed more extensive sequencing targeting all exons by PCR or cDNA amplification demonstrating complete sequencing of the coding regions [ 18, 19].
We hypothesize that more extensive sequencing of the vegetative libraries would lead to the identification of homologs of CTB genes such as those identified in C. nicotianae.
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