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More extensive microarray and proteomic studies in rodents and humans would help to characterize the pathways disrupted by atrazine.
The authors proposed that more extensive microarray analyses might reveal additional markers that would improve the predictive value of microarray profiling.
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Studies from our laboratory and from others have used more extensive microarrays than those used in the two SLE studies described above to characterize the broad gene expression profile operative in the peripheral blood of patients with SLE [ 3- 6].
Our findings suggest that a more extensive, replicated microarray analysis involving higher numbers of air pouch membranes may be a powerful tool to identify genes that are differentially active in various aspects of inflammation and innate immunity.
Finally, I suggest future paths for more extensive application of microarray to ecotoxicology.
With the new EST database, a new, more extensive 32 K cDNA microarray has been developed to help assess gene expression patterns in salmonids.
The complete gene sequences for all of the genes represented on the microarray, the sequences of the probes printed on the microarray, expression profiles of all of the genes on the microarray and more extensive BLAST annotation are available as GEO series accession number GSE6855.
As previously demonstrated for microarray approaches, more extensive explorative capture probe sets could recover rare sequences, leading to the detection of many uncharacterized microbial populations.
A more confident analysis would require much more extensive sequencing data, or the use of microarrays.
The CjFur and CjPerR transcriptomes characterized in this study were compared to those previously identified using microarray profiling and found to be more extensive than previously understood.
A more extensive testing for other known pathogens by using virochip microarrays, metagenomics, and direct testing for pathogenic bacteria will help determine whether BuVs alone can cause diarrhea.
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