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Sensitivity levels obtained with the current assay were sufficient for a functional assay and were improved with the use of a more extensive amplification protocol.
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The latter protocol was used to test whether increased sensitivity of the assay could be achieved with a more extensive PCR amplification.
These data suggest that the duration and scope of the recent Mexican epizootics were limited by lack of equine amplification characteristic of previous, more extensive VEE outbreaks.
Recent publications showed more extensive sequencing targeting all exons by PCR or cDNA amplification demonstrating complete sequencing of the coding regions [ 18, 19].
Although preliminary reports of preclinical studies have shown that anti-EGFR treatment in amplified tumours does not significantly reduce the progression of breast carcinomas, more extensive studies are required that will accurately evaluate EGFR amplification by ISH and EGFR overexpression by IHC in TNBC.
CNV analysis initially could begin with CNVs reported other PCD cohorts, followed by more extensive full exon analysis using array CGH or multiplex ligation-dependent probe amplification (Stuppia et al. 2012).
Co-infection with ONYX-017 + Adeno-2B6 also increased CPA-dependent bystander cell killing, most likely by ONYX-017 increasing Adeno-2B6 genomic amplification expression as well as potential spread, resulting in more extensive CPA metabolism and increased 9L/lacZ cell death; however, in all cases bystander cell death was less extensive than that seen with Adeno-2B6/p35.
In a step further we now evaluated for the first time in a more extensive systematic approach five different DNA extraction methods in combination with five different amplification systems for their sensitivity to detect C. trachomatis in SF.
French influence was more extensive.
"This is much more extensive".
(Drop-off locations are more extensive).
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