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More experiments should be done in GalN/LPS-treated AMPKα1LS−/− mice.
Therefore, more experiments should be done to prove which one might lead to a better result.
However, more experiments should be performed including genetically inhibiting autophagy, such as siRNA against Atg7 or beclin1 to further confirm autophagy-dependent protective effect of M1 on ActD/TNFα-induced cell death.
This means that much more experiments should be performed to validate the bias.
Also, more experiments should be implemented to verify the performance of related technologies in the framework.
The influence factors of the permeation times maybe included solvent, concentration of the compounds, pH value, et al. More experiments should be done in future analysis.
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Additionally, these results suggest that more controlled experiments should be conducted to more fundamentally evaluate particle emissions from a wider arrange of desktop 3D printers.
If it seems reasonable to perform more experiments, one should return to step (2), performing experiments at the condition indicated by the optimal experimental design techniques [ 38, 39].
Thus more rigorous experiments should be conducted to determine biomarker cut-off optimization and calculation of ROC curves.
More specific experiments should be performed for a better understanding of the effect of Sal B on bone healing.
However, a large group of patients with long-term follow-up is needed, and more cellular experiments should be performed, to confirm this conclusion.
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