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The codon usage of essential genes suffers from more evolutionary constraints than non-essential genes.
This suggests that the former sequences represent a gene that is subject to more evolutionary constraints, similarly to MSMP in vertebrates.
Once all these synteny blocks have been detected, we showed that POGs are subject to more evolutionary constraints than orthologs outside synteny groups, whichever the taxonomic distance separating the compared organisms.
TvPirin, on the other hand, as a transcription co-factor, probably interacts with other proteins with specific conformations for DNA binding, and thus would be subject to more evolutionary constraints in order to maintain specific protein-protein interactions, which might have led to the much lower molecular diversity observed here.
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This suggests that e/aRF1 is under more evolutionary constraint at the sequence level than e/aDom34p.
This suggests that "housekeeping" HyPRPs may be subjected to more rigorous evolutionary constraints (see Figure 2).
This suggests that genes belonging to gene sets are globally under more severe evolutionary constraints (table 1).
Taken together, these observations suggest that Mkrn4 is subject to more relaxed evolutionary constraints than Mkrn1 and Mkrn2, with possible development of new general and lineage-specific functions.
Recent studies observed that MFS was strong for more abundant proteins, resulting in stronger evolutionary constraints of more highly expressed proteins [ 26, 27].
On the other hand, co-expressed multi-interface hubs evolve at a significantly lower rate, indicating that hubs with more permanent interfaces are subject to higher evolutionary constraints and thus evolve more slowly.
Focussing on genes grouped according to function, we ask whether a functional category harbours an excess of nonsynonymous polymorphic coding positions showing specific signatures of constraints, and more interestingly recent changes in evolutionary constraints specifically in the human lineage.
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