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Analysis over several months would probably show more even coverage.
Supplementary Figure 11 TAPS resulted in more even coverage and fewer uncovered positions than WGBS.
On a more practical point, Emina and Gail's heat their syrup and fat together before stirring it into the mixture, which gives a more even coverage.
Sunscreen lotions and creams provide more even coverage than sprays and tend to be absorbed less through the skin, which is what we should aim for.
This parameterization is better in the sense that the points obtained by using the NBI method produce a more even coverage of the Pareto curve and this coverage does not miss the interesting middle part of the Pareto curve.This useful property, provides an incentive to create a new method.
We applied TAPS to the whole-genome mapping of 5mC and 5hmC in mouse embryonic stem cells and show that, compared with bisulfite sequencing, TAPS results in higher mapping rates, more even coverage and lower sequencing costs, thus enabling higher quality, more comprehensive and cheaper methylome analyses.
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The purpose for amplifying the three different populations of cDNA fragments was to ensure more even sequence coverage along transcripts by reducing coverage bias towards the 3′termini of transcripts caused by the transcription of truncated mRNA during first-strand cDNA synthesis.
Fragmentation of RNA and/or cDNA has been used previously [6] to achieve more even sequence coverage throughout the length of transcripts.
The SMRT technology also produces more even sequence coverage of the high mol% G + C DNA found in actinomycete genomes than other NGS platforms [ 8].
These CV values were lower than the lowest CV value reported in the benchmarking study referenced above (0.54), indicating more even read coverage in our samples [ 60].
Although the PCR-free approach has improved read distribution and produced more even genome coverage, it requires large amounts of starting DNA material, which is difficult to obtain especially from clinical isolates.
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