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The process involved hundreds of cascades, in which corrosive uranium hexafluoride diffused through gaseous barriers, becoming progressively more enriched at every stage.
In vivo ChIP revealed that H3K9 acetylation is more enriched at the Fabp7 promoter in the wild-type than in COUP-TFI−/− newborn brains (Figure 4B), consistent with a mechanism that COUP-TFI regulation of Fabp7 is associated with higher chromatin accessibility and transcriptional activity.
Notably, the binding sites were more enriched at the vicinity of (∼70 nt upstream) of 3′ splice site than at 5′ splice site.
Conversely, VDR, TFAP2A, TFCP2, BHLHE41, and NFIC motifs were more enriched at poised HSEL than active HSEL (Additional file 2: Table S9).
Consistent with the above semi-quantitative PCR results, RAP2.1 was more enriched at the D1 fragment (about 9.25% of input) than D2 (about 3.16% of input), indicating that RAP2.1 binds to D1 fragment with higher efficiency than D2 fragment.
There are two functional implications of Hmt1's enrichment across the various transcriptional frequency gene classes – first, the highly transcribed gene class contains many of the ribosomal protein genes that encompass many intron-containing genes and Hmt1 has been shown to be more enriched at the 5'-end of these genes.
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All genera (with n>1) present at both localities become significantly more enriched in 18O at the interglacial site (Hemiauchenia, p = 0.0001; Odocoileus, p<0.05; Platygonus, p<0.01) with the exception of Tapirus and Equus, the latter of which lacks statistical significance potentially due to sample size.
All genera (with n>1) present at both Leisey 1A and Inglis 1A are significantly more enriched in 13C at the interglacial site (Equus, p<0.05; Hemiauchenia, p = 0.0001; Odocoileus, p = 0.001; Platygonus, p = 0.0001) with the exception of Tapirus.
The whole and acid-insoluble samples are more enriched in the peak at 1.5 ppm than in the oxygenated region (as they have larger negative PC1 scores).
The C18 samples (with positive PC1 scores) are more enriched in the oxygenated region at 2.4-3.2 2.4-3.2 this region has ppmitive PC1 loasings) this in the peak at 1.5 ppm.
Just as for Neurl4, GFP Neurl4 was concentrated apically, although more clearly enriched at two foci above each nucleus (Fig. 4B,C).
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