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For instance, the reporter might have a lower barrier to activation and be more efficiently transcribed relative to the endogenous enhancer.
Genes that are negatively supercoiled are generally more efficiently transcribed [ 9,10] but topoisomerase inhibition studies [ 11,12,13,14] indicate that the accumulation of excessive positive or negative supercoiling will repress transcription.
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Rarer targets can be more efficiently reverse transcribed into cDNA this way; Promega technical notes suggest the use of 3.125 U/ l for reverse transcription instead of 1.25 U/ l in this regard.
Therefore, there is either an exceedingly large number of pre-miRNAs in these datasets or, more plausibly, most of these candidates are not efficiently transcribed but could otherwise be recognized as miRNA precursors.
The wheat P450 cDNAs were efficiently transcribed, but no protein or activity was found.
As shown in Figures 1A and 1C, all templates were efficiently transcribed (compare lanes 1 and 2).
These findings support the idea that uAUG genes are less efficiently transcribed than uAUG-less genes.
The PTH gene was efficiently transcribed, processed and translated to mature immunoreactive PTH.
This suggests that the exon 1 3 mRNA is either not efficiently transcribed or unstable.
This insertion could be assigned as a most efficiently transcribed LTR region within SORF2 region of MDV [ 4].
This "replicational selection" should guarantee that genes on the leading strand, particularly the essential ones, are efficiently transcribed [ 5- 8].
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