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This is supported by the observation of Patil et al. [46], who examined internalization of cerium oxide nanoparticles and found that negatively charged nanoparticles more efficiently internalized into adenocarcinoma lung cells than the positively charged.

Confocal laser scanning microscopy observation and flow cytometry analyses indicated that PEGylated RES conjugates were more efficiently internalized into cells, released drug into cytoplasm.

NP1/DOX with faster acid-triggered drug release can be more efficiently internalized by two-dimensional (2D) cells and three-dimensional (3D) multicellular tumor spheroids (MCTS), and lead to improved antitumor efficiency.

However, it has also been reported that PM proteins, with short polyubiquitin chains, are more efficiently internalized [ 19, 52- 54].

In addition, previous results from our group have shown that opsonized virions are more efficiently internalized by DCs via receptor-mediated endocytosis 12.

On the other hand, the cell penetration properties of cyclic frameworks could also play a role in the different route of entry into cells, as D'Souza et al. [ 60] have recently shown that MCoTI-II is more efficiently internalized into cells than SFTI-1.

Similar(54)

In porcine kidney cells, (-IRE -DMT1 isoforms have been shown to be more rapidly and efficiently internalized upon metal exposure when compared to (+IRE -DMT1 isoforms [39].

The strong fluorescence from the cytoplasm confirmed that the QDs were efficiently internalized by the cells.

In vitro studies showed that protamine nanocapsules were efficiently internalized by macrophages without eliciting significant toxicity.

The obtained results showed that neutral PC-liposomes are the most efficiently internalized by HeLa cells.

A2 was efficiently internalized into both cell lines via at least two different mechanisms.

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