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Monoolein produces signals that correspond more closely to the calculated amount, possibly because it is more soluble and therefore more efficiently cleaved by the lipase.
However, all of them were substantially more efficiently cleaved by caspase-3, suggesting a minor, if any, physiological importance of caspase-6.
In mammalian cells, m7G-capped transcripts were demonstrated to be more efficiently cleaved at the poly(A) site than incompletely capped or uncapped transcripts [ 88, 95].
While most were also processed following incubation with other caspases, the translation initiation factor EIF4B was one of a minority which were more efficiently cleaved by caspase-2 [ 41]; however, the significance of this cleavage event remains to be defined.
In the hope of generating more caspase-2-selective reagents, we evaluated custom fluorogenic peptides bearing sequences that this study revealed were more efficiently cleaved by caspase-2 than caspase-3.
As a result, l-Bpa is much more efficiently cleaved compared to l-Bip.
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The cyclization product more potently suppresses the cellular immune response than its linear counterparts and is efficiently cleaved by trypsin.
Interestingly, the G68P mutant, which was almost completely defective in cleaving HA-MBP-RseA148, efficiently cleaved HA-MBP-RseA LY1 148 HA-MBP-RseA LY1 148 HA-MBP-RseA LY1 148d more profound effects on the cleavage of HA-MBP-YqfG than on the cleavage of the other 2 model substrates.
When used alone, neither ASP nor DPPIV efficiently cleaved synthetic immunotoxic gluten peptides.
PARP was also efficiently cleaved, despite weak endogenous caspase-8 activation (Fig. 8C).
Among the four substrates, Z-YPQ-pNA was most efficiently cleaved by enzymes contained in dental plaque (Figure 1A).
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