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A relatively small decrease in the SOS-mediated GTP-analog off-rate and consistently an increase in EGF-stimulated activation however suggested that this mutant may be more efficiently activated.
We currently have no explanation for this, but it is tempting to speculate that they are formed prior to activation by UBE1 and represent forms of NEDD8 much more efficiently activated by UBE1.
The modified TGF-β1 produced from hChonJb 7 cells had activity comparable to fully active rhTGF-β1, suggesting that modified TGF-β1 preprotein was more efficiently activated than WT preprotein.
It is also known that Kss1 is more efficiently activated than Fus3 when Ste5 is not membrane recruited [2].
Because cell-bound PLG is more efficiently activated to PL, we first examined the expression of PLG in PBMC from patients with psoriasis by flow cytometry [22].
Pam2Cys12 and Pam2Cys17 more efficiently activated the ELAM promoter (>300 fold over the control) with TLR2 and 6, than with TLR2 alone (Fig. 4A).
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In larvae lacking buffy, decreased cellular energy (discussed further below) might more efficiently activate ATG1/ATG13, possibly mediated through AMPK.
PAMs are also capable of promoting neuroprotection in striatal neurons from a mouse model of HD, BACHD, and one of the tested PAMs, VU1545, more efficiently activates Akt and promotes neuroprotection in BACHD neurons than in wild-type (WT) neurons.
Since EWS/FLI-1 or EWS/GAL4 can more efficiently activate reporter constructs regulated by HTLV Ets DNA-binding or GAL4 DNA binding elements than similar FLI-1 proteins (May et al, 1993b; Bailly et al, 1994), it has been suggested that EWS/FLI-1 could act by overexpressing genes normally regulated by FLI-1, resulting in tumorigenesis.
Some compounds appear to more efficiently activate human compared with rodent PPAR-α in vitro, as was demonstrated for the synthetic polyunsaturated fatty acid 5,8,11,14-eicosatetraynoic acid in transfected human liver cancer HepG2 cells (Mukherjee et al. 1994) and for perfluorobutane sulfonate in transfected COS-1 monkey kidney cells (Wolf et al. 2008).
TIGAR-hBS2, the more efficient of the two human p53-binding sites, is efficiently activated by either human or mouse p53.
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