This investigation has been motivated by previous observations suggesting that the mRNA degradation following binding of miRNA is more efficient for transcripts carrying multiple target sites for miRNAs [32] [34].
This process reduces oversampling of abundant transcripts such those derived from the chloroplast or nuclear genes involved in photosynthetic processes (e.g. rbcL, rbcS, cab), and more efficient detection of transcripts expressed at low levels in specific tissues.
The first is overkill: faced with the seemingly simple challenge of segregating nascent transcripts from DNA, why would evolution have devised as elaborate and seemingly problematic a mechanism as the spliceosomal system, rather than a simpler and presumably more efficient TREX-like transcript-coating mechanism?
The latter might be due to the higher abundance of the transcript, or due to more efficient amplification of the transcript-3 primer set.
To induce termination, Nrd1 needs to associate with initiating polII, such that the Nrd1 Nab3 complex is more efficient at terminating short transcripts than longer ones [ 83, 84].
Overexpression of eIF4A could increase the unwinding of mRNAs with highly structured 5′ UTRs and allow more efficient translation of these transcripts.
It could be argued that when large changes are required, it would be more efficient to generate one antisense transcript that can control multiple pathways than to generate multiple individual transcripts.
This will allow the more efficient identification and characterization of transcript specific RNA editing accessory proteins.
This may allow the more efficient identification and characterization of transcript specific RNA editing accessory proteins.
As already demonstrated, siRNAs targeting early transcripts (among them the pTP and DNA polymerase mRNA-targeting siRNAs) were much more efficient than those targeting late transcripts (i.e., those coding for the hexon protein and the viral protease).
