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The aim of this study was to develop a more efficient targeted gene deletion strategy for Clostridium.

Therefore, introducing in the in vivo studies promising synergistic to TRAIL agents such as Quercetin that have been proved efficient in vitro in our lab (Psahoulia et al, unpublished data) may lower cytotoxicity by reducing even more TRAIL concentrations leading to more efficient targeted therapies.

Many of the searchable keyword domains in AcuTrials® mirror the 2010 revised Standards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA) [ 22] checklist of essential reporting items, allowing researchers to perform more efficient, targeted literature searches based on these important components.

Therefore, in order to design more efficient targeted therapies, we suggest that 3D cell culture models should be employed in cancer stem cell research.

Increased monitoring could identify hotspots where subtype H5N1 is circulating, leading to more efficient targeted vaccination of poultry, and could pinpoint prefectures at high risk for a reassortant virus.

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Knowledge of the significance of hotspot zones would enable more accurate calculation of total farm emissions and more efficient targeting of N2O mitigation strategies.

In health care, the use of such feedback loop enables real-time analysis of patient databases and could permit to optimize clinical care leading to more efficient targeting of tests and treatments and vigilance for adverse effects (i.e. dynamic clinical data mining) Cross-validation Statistical technique for assessing how the results of an analysis will generalize to an independent data set.

The main idea is the use of feedback loops to enable real-time analysis of patient databases, allow the optimization of patient's care and lead to more efficient targeting of tests, treatments and vigilance for adverse effects (e.g. "Multiparameter Intelligent Monitoring in Intensive Care" (MIMIC) [2].

This may lead to more efficient targeting of the hypoxic tumor environment in the future.

A clearance system is therefore desirable with this antibody-enzyme conjugate or a more efficient targeting system is required.

This could be overcome by more efficient targeting of the cytokine to the thymus, rather than systemically using high doses.

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