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Here, we report that unlinkase activity can be detected using this assay and confirm previous reports that the full-length virion RNA is a much more efficient substrate than a VPg-nonanucleotide substrate.
Since the contaminating natural amino acid is a more efficient substrate than the N-methyl analog and is at a much lower concentration, most of the natural amino acid became attached to the tRNA and was removed by EtOH precipitation [54].
Based on these data, we conclude that not only is full-length poliovirus virion RNA a more efficient substrate for unlinkase activity than the VPg-nonanucleotide substrate, but the shorter substrate forms an intermediate that cannot be cleaved by unlinkase, confirming that the length of the RNA plays a role in the activity of this enzyme.
Possibilities include more efficient substrate gut absorption and less energy expenditure.
Furthermore, ECyd is a more efficient substrate for UCK2 than for UCK1.
Glucose is a more efficient substrate for ATP production than free fatty acid (FFA).
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The PmeI digestion releases the Ad ITRs from almost the complete plasmid backbones turning them into much more efficient substrates for the initiation of Ad-dependent DNA replication than undigested templates.
Using PCT, important outstanding questions in the area of recalcitrance and deconstruction of biomass can be clarified, which will provide new insights to obtain more efficient substrates and pretreatment processes.
Therefore, one could hypothesize that females may be more efficient in substrate utilization even though they have reduced mitochondrial biogenesis.
Grimaud et al. [ 50] suggested that structural changes in amylopectin, due to the loss of SBEIIb, could offer a more efficient glucan substrate for binding of SBEI, SBEIIa, starch phosphorylase and SSIII.
With glucose as the more energetically efficient substrate, the healthy heart is able to switch to glucose under conditions of stress, such as ischaemia, pressure overload or in heart failure.
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