According to Landis and Koch's [ 17] scale there was a substantial agreement between the methods, to NPA's favor, and NPA was significantly more efficient collecting epithelial material approximated by quantification of ACTB.
More accurate and more efficient forward and inversion problems for improving the quantification accuracy and reconstruction speed are also being investigated.
Quantification of transduction efficiencies revealed that ShH10 was approximately 62% more efficient at infecting Müller cells relative to AAV2, infecting 22% vs. 14% of total Müller cells respectively in transverse retinal slices (Fig. 3b).
Such applications of RNA-Seq require, however, analysis tools (mapping, quantification, etc) that are order of magnitude more efficient than currently existing ones.
Interestingly, quantification (Fig 2D; supplementary Fig S4D online) shows that USP4 D1D2 without insert is more efficient at degrading both di-Ubs than the complete catalytic domain.
This chapter focuses on convenient computerized methods for the quantification of ISH results, as these are now very commonly used and are usually more efficient than manual methods.
Similarly to what observed with the ABCA4 and MYO7A transgenes, quantification of EGFP expression (Fig 2F) showed that dual AAV TS and hybrid AK approaches are more efficient than AAV OZ.
