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Compared earlier syntheses, this method offered a reduced number of reaction steps, milder reaction conditions, and a more efficient purification process.
Therefore, there is an increasing demand for a more efficient purification method that is applicable for the large-scale production of pure fullerenes.
SMB, therefore, has the potential to replace single column discontinuous chromatography in order to design more efficient purification trains for production of cell culture-derived influenza vaccines.
Due to the amount of compounds involved in the biodiesel production, liquid liquid equilibrium data are essential in order to predict the proportions in which these compounds exist and subsequently proceed with a more efficient purification and aid in the design of the reactor and ponds.
The quantity of a ∼100 kDa protein increased substantially as a consequence of RNase treatment, suggesting that disruption of endogenous RNPs allows more efficient purification of this factor.
Thus the more efficient purification of Rrp41 in caf40Δ and not4Δ in one hand and the co-purification of Not5 with Rrp41 in other hand are correlated with the release of Rrp41 from large soluble structures.
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His-tag affinity purification was found to be more efficient than purification via GST-tag for some proteins.
Therefore, treatment at pH 3.5 was apparently more efficient for purification, without detectable SELP losses being observed during the process.
Addressing this challenge necessitates more and efficient purification plants which can be realised by optimal design at conceptual stage.
Alternatively, YK5 could be directly immobilized on a solid support (i.e., YK5-beads) resulting in a potentially higher localized concentration of ligand; this may enable more efficient affinity purifications of Hsp70 complexes.
However, we recently showed that the purification of rCTB with a His-tag from the culture supernatant of L. casei was 20 times more efficient than the purification of rCTB without a His-tag [ 5].
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