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Moreover, recent experiments suggesting that the 1918 virus had a more efficient polymerase than current circulating H1N1 strains [32] would further support the results here, since this would have caused the 1918 virus to present even more immunostimulating motifs to PRRs.
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Moreover, DNA polymerase λ is more efficient than DNA polymerase β to fill this oxidized single-nucleotide gap.
For other steps, TAQ DNA polymerase was used due to a more efficient amplification using this polymerase.
This, in turn, results in increased stability of the misaligned substrate, allowing for more efficient extension by the polymerase.
Indirect labeling of RNA for microarray purposes via incorporation of aaUTPs was preferred over direct incorporation of fluorescently labeled nucleotides, as it has been proved to be more efficient with T7 RNA polymerase based methods [ 12].
The advent of protein generation (translation) can speed up evolution by replacing existing abiotic replication and transcription processes by more efficient protein enzymes, for instance RNA polymerases.
A virus's polymerase enzymes are often much more efficient at making DNA and RNA than the host cell's.
Such a specific polymerase would have been much more efficient, as it would not waste its time making non-functional polymers.
Therefore, Φ29 DNA polymerase has a lower error rate, more efficient amplification reactions and appears to be more suitable for additional sequencing studies.
As already demonstrated, siRNAs targeting early transcripts (among them the pTP and DNA polymerase mRNA-targeting siRNAs) were much more efficient than those targeting late transcripts (i.e., those coding for the hexon protein and the viral protease).
Studies have shown that addition of forward primers after the reverse transcription reaction and before activation of Taq DNA polymerase prevents the formation of primer-dimers and results in more efficient amplification [16].
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