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In any case the new, more efficient mutant spreads, and the metabolic system itself also benefits.
For a more efficient mutant screening, a Tc-PCR with the SDM-generated mutants as templates and the same mutagenic primer pairs was designed to replace the traditional Dpn I digestion.
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In order for protein engineering to design more efficient mutants of TlL for specific applications, it is important to characterize its interfacial catalysis.
They found that the more efficient a mutant was at turning on genes, the more time it spent flipped into the active shape, they report in the 23 March issue of Science.
The difference is about 5°C, certainly closer to the wild and more efficient to mutant enrichment.
Interestingly, the presence of AmpA strongly interferes with the more efficient sma− mutant strain's chemotaxis and impairs cell movement.
In this scenario, the native HAHB4 protein would be more efficient than the mutant proteins H4WCT and H1WCT in exerting this inhibitory activity.
Accordingly, from an evolutionary point of view, it becomes more and more efficient for the mutant to increase its immune escape than its transmissibility in order to invade the population.
Whether a pro-folding chaperone, like DNAJB1 and Hsp70 (49), would be more efficient in restoring mutant Parkin activity or whether the anti-aggregation activity is a prerequisite for this recovery remains to be tested.
This suggests that the initial difficulty of assembling three different mono-active replicators in the same metabolic neighbourhood is circumvented by evolving bi-active ones that are later excluded by their own, more efficient mono-active mutants.
The hFXNG130V mutant appears more efficient to rescue lethality than the hFXNI154F mutant although neither clone completely rescues the phenotype.
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