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Packed with glass beads, the hot-zone reactor resulted in more efficient initiation and film growth.
No substitutions in the aa (9 11) region were detected that could explain a more efficient initiation of synthesis of core+1/ARFP from this site [19].
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A more efficient first initiation site in human COMT mRNA leads to the production of more membrane bound MB-COMT than soluble S-COMT in human brain [30] which, despite having a lower enzyme capacity, has a higher affinity for dopamine and other neurotransmitters [31], [32], [33].
Whether the accumulation of 5′ leader ribosome density is a mechanism that assists in this process or is it merely a byproduct of more efficient ribosomal initiation on these templates remains to be tested.
The hypothesis explains why a long pre-AUG poly(A) leads to more efficient translation initiation than a short one when PABP is absent, and why pre-AUG poly(A) is short in the early genes but long in the late genes of vaccinia virus.
The PmeI digestion releases the Ad ITRs from almost the complete plasmid backbones turning them into much more efficient substrates for the initiation of Ad-dependent DNA replication than undigested templates.
This finding suggests that both substances are more efficient in preventing cancer initiation than delaying its progression.
In PTEN-null mice, there is a preferential expansion of basal cells relative to luminal cells [ 18], and these cells demonstrate more efficient capacity for cancer initiation relative to luminal cells.
The helicase activity of eIF4Ac is ∼20-fold more efficient than eIF4Af and during initiation, eIF4Af likely cycles through the eIF4F complex [11].
In contrast, when translation initiation is more efficient in groups MFE2 MFE4, rProt increases significantly with ITE, with the simple linear model consistently accounting for about 17% of the total variation in rProt.
This observation and the fact that colospheres are more efficient than spheroids in the initiation of tumour growth and metastasis in immunodeficient mice led us to study the membrane expression of two cancer stem cell markers, CD133 and CD44, in XenoCT320 colospheres.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com