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Exact(33)
Thus, we used electroporation for more efficient gene expression instead of chemical transfections and increased the efficiency up to 65%.
Two papers describe the optimization of RNAi molecules for more efficient gene silencing.
We anticipate that this method will also aid the design of more efficient gene carriers.
We have found that retroviral vectors containing the complete packaging signal allow more efficient gene transfer into a variety of cell types.
Understanding the interaction of polyarginine and polyethylenimine with the plasma membrane may assist in designing more efficient gene delivery systems.
More efficient gene synthesis techniques and the decreasing costs of commercial oligosynthesis are making this approach both simpler and less expensive to perform.
Similar(27)
With the emergence of more efficient gene-editing techniques, e.g., CRISPR/Cas (Cong et al. 2013), an RHS-type approach could also be feasible in the near future for human cells, which should enhance the detection of functional alleles for phenotypes with medical implications (e.g., drug resistance and cancer development and progression).
Consequently, the genomic homeostasis mechanisms that naturally exist to combat dietary and environmental insults in the colon of the normal rat appear to be dysregulated by AOM resulting in a cellular switch through p53 signaling to more efficient genes associated with the apoptotic response, a genetic response that is also reflected histologically.
Rational design of more efficient polymeric gene carriers will be possible only with mechanistic insights of the rate-limiting steps in the non-viral gene transfer process.
This study demonstrated that CRISPR/Cas9 was more efficient at gene knock-ins in domesticated animal cells than ZFNs and TALENs.
The rational design of more efficient nonviral gene carriers will be possible only with better mechanistic understanding of the critical rate-limiting steps, such as nanocomplex unpacking to release DNA and degradation by nucleases.
More suggestions(15)
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