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The α6 subunit can form a functional receptor when co-expressed with β4 in vitro, but more efficient expression ensues from incorporation of a third partner, such as β3 242.
These results indicated that the expression of SlaR resulted in more efficient expression of slaAB under the control of P C than P A or P R, 394% improvement of the integrated activity of α-ALS and α-ALDC was detected in MG15.
The shorter oligonucleotides required (45 bp versus 64 bp for the shRNA construct) also makes this vector a more efficient expression vector for multiple siRNAs.
pGL4.20 was chosen as it is optimized for more efficient expression in mammalian cells, compared to previous generations of luciferase constructs, and contains fewer transcription factor consensus sequences such that background luciferase expression is reduced.
Some codons of this synthetic luciferase 2 have been optimized for more efficient expression in mammalian cells (GenBank AAV52869.1).
In respect of these side effects, the more efficient expression of the synthetic ntro gene used in this study allows application of lower CB1954 concentrations in vitro.
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Compared to conventional plasmids, minicircle DNAs devoid of plasmid bacterial sequences are superior as non-viral DNA vector for multiple reasons: (a) relative safety due to the reduced numbers of inflammatory unmethylated CpG motifs; (b) more efficient transgene expression due to its reduced size; and (c) more robust and persistent transgene expression [16], [23], [24].
Thus, we used electroporation for more efficient gene expression instead of chemical transfections and increased the efficiency up to 65%.
Therefore we hypothesized that generation of Ad vaccine vectors expressing cytotoxic genes, such as HIV-1 env, would be more efficient if expression of the transgene was down-regulated during Ad rescue.
In this more efficient CF expression system, a RM (reaction mixture) containing all the high molecular weight compounds of the reaction is separated by a semi-permeable membrane from a FM (feeding mixture), providing fresh low molecular weight precursors for the reaction.
This knowledge would be of substantial benefit to the development of more efficient shRNA expression systems for cross-species RNAi applications.
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