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However, the latter step remains mostly elusive, and, in order to better control differentiation and design more efficient differentiation strategies, the cellular mechanisms behind different pluripotency stages that mimic embryonic development are being actively addressed.
Toward this end, there is a large number of developmental and β-cell functional studies that could guide the design of more efficient differentiation protocols, predominantly during the late stages.
We are currently seeking the better method enabling more efficient differentiation.
C2Q16 cells are a previously described subclone of C2C12 cells [36], characterized by slower but more efficient differentiation, compared to the parental cells.
Downregulation of Runx1 resulted in a more efficient differentiation (Figure 3A); both the expression of muscle markers and the proportion of multinucleated cells were higher in Runx1-depleted cells (Figure 3A and Figure S4A).
In conclusion, the present study demonstrated that overexpression of NTRK1 facilitates more efficient differentiation of NSCs into cholinergic neuron in response to NGF treatment.
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As shown in Supplementary information, Figure S2C and S2D, we found that monolayer-based differentiation resulted in more efficient endothelial differentiation and a higher yield of ECs than EB-based differentiation, and therefore all subsequent experiments were performed using the monolayer differentiation protocol.
In addition, treating iCPC with Wnt inhibitor IWP2 facilitated even more efficient cardiac differentiation.
Our results showed that the more crosslinked FS membranes enabled a more efficient myoblast differentiation in myotubes.
Conceivably, more efficient cardiogenic differentiation within the environment of infracted heart may have yielded higher Connexin 43 expression and maturation of ion currents in engrafted cardiomyocytes resulting in improved functional in vivo integration.
This indicated a more efficient granulocytic differentiation response to RA treatment of NB4-siAgo2 cells compared with control cells.
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more functional differentiation
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