Although the ELISA appears simpler, safer and more efficient, the assay is less sensitive in detecting low values of rabies virus neutralizing antibodies than neutralization tests.
TILLING, CAPS markers, or a more efficient SNP assay can be used as tools to track the inheritance of these alleles in the breeding process.
More efficient diagnostic assays will help with both clinical diagnosis and disease surveillance.
Cells treated or not with 50 ng/ml KGF, the concentration reported to be more efficient in this assay [34], were allowed to migrate from the edge of the wound for 24 h.
Fortunately, more efficient and reliable assays based on PCR and DNA sequencing have become commonplace as part of the identification process of novel species (and differentiating closely related species).
Our original methodology used blotting and detection with an oligonucleotide to increase sensitivity [ 7] but this is no longer necessary with the more efficient RT-PCR assays presented here, in particular because of the use of a hot start Taq polymerase.
The results indicate that HRM analysis is useful as resequencing in medaka [ 26] and more efficient than CEL I assay in mammals [ 40].
To make the assay more efficient, a cohort of normal samples was used as a standard, permitting a threshold cut-off value (termed Z) to be determined: values above this were deemed to show DS cases (Fig. 2).
In our present study, we report a BALF-GM assay sensitivity of 80% (8 of 10 cases) and a serum-GM assay sensitivity of 40% (4 of 10 cases) for the diagnosis of noninvasive pulmonary aspergillosis, suggesting that the BALF-GM assay is more efficient in diagnosing this infection than the serum-GM assay.
To fabricate more efficient and reliable bio-assays, it is essential to develop a method to immobilize probe ssDNAs with a more uniform coverage and in a geometric arrangement which minimizes non-specific bindings.
