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This strategy yields functional variants that retain the original activity at higher frequencies, suggesting a new strategy for protein engineering that incorporates a more divergent sequence exploration beyond that limited to point mutations.
Within the transcript body, highly conserved patches are interspersed with regions or more divergent sequence.
Discontinuous MegaBLAST is claimed to be able to identify more divergent sequence similarity than BLAST.
In addition to these changes, PpHxk11 has several more substitutions in the conserved regions, consistent with its generally more divergent sequence.
One protein, encoded in Francisella philomiragia subsp. philomiragia ATCC25017, has a more divergent sequence but still returns a significant Pfam hit, while a sixth small protein, from Haemophilus influenzae 10810, has a divergent sequence and does not yield a Pfam hit.
For a given TA-PLM, the number of promoters containing it depends on both the distance from the seed, i.e. the number of substitutions between the given PLM and the seed-PLM and the existence or not of a more divergent sequence.
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The sequence divergence comparisons (Table 1) consistently show that the enriched library displays more divergent sequences (higher mean) than the control library using as reference the zebrafish genome.
The presence of defective fragments of Bari and 412 elements in the sequenced genomes of both species and the presence of two more divergent sequences of the retrotransposon 412 in populations of D. melanogaster (as shown in the network) indicate that both elements were present in the common ancestor of these species and were inherited by both species.
This may simply be due to more divergent sequences of mitochondrial proteins common to the Eukarya or that these antibodies detect mitochondrial proteins unique to the Apicomplexa.
Hox-proteins with more divergent sequences might be of greater interest for evolutionary studies or when trying to find common, shared or even divergent amino acid motifs responsible for the differences in function.
This type of clustering allows for more divergent sequences to be grouped.
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