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The AT1R antibody generally induced a more distinct staining than the AT2R antibody.
As our protocol consistently yielded stronger and more distinct staining for all the antibodies, the final staining for all the 175 cases with SMA and a double immunostaining with additional markers in selected cases (see below) were performed with our published protocol [ 26].
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Fluorescence microscopy showed more distinct and intense albumin staining in spheroids with cell crosslinker than that without crosslinker.
Even though it is difficult to evaluate the intensity of the Bcl-2 expression, a more distinct cytoplasmic Bcl-2 staining was noted in plasma cells from pSS salivary glands compared to plasma cells from subjects with chronic inflammation and normal salivary gland tissue.
With regard to the evaluations of HGM, MUC6, MUC2 and CD10 staining, distinct staining in more than 5% of the tumour cells was recorded as positive immunoreactivity for the relevant marker.
We found that either there was no/sporadic nuclear staining or a distinct staining intensity in more than 5% of all tumour cell nuclei regardless of the cytoplasmic-staining levels.
GPER was positively stained in a cytoplasmic pattern, and 59 tumor samples (41.8%) were considered to be stromal fibroblast GPER-positive based on distinct staining of 10% or more of the fibroblasts (Table 2).
Confocal images show localization of both class1 and class2 on vessels structures (Figure 6A, B) and confirmed our finding that the two scFv classes have a distinct staining pattern, with class1 binding the more external layers and class2 the more internal layers.
Distinct staining of the nucleus in more than 10% of tumor cells was recorded as positive for ER and PgR, and strong membrane staining in more than 10% of invasive lesions was recorded as positive for HER2.
The staining obtained using the RNAscope method appeared to be more distinct and to reflect more accurately the subcellular localization of the transcripts, compared to the staining in the standard and the optimized whole-mount FISH, which appears more aggregated and more diffuse.
But the parties are ideologically more distinct.
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