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CSF samples were selected from archived specimens obtained during two observational studies conducted at the University of California San Francisco (UCSF), one following the course of a cohort of HIV-infected subjects with periodic evaluations that included lumbar punctures (LPs), and the other study more directly evaluated CSF responses to initiation of cART [ 5, 6].
However, hypoxia can also be more directly evaluated by PET using [F]fluoromisonidazole (FMISO), an imidazole compound that is specifically trapped within hypoxic cells.
In another study that more directly evaluated the ability to estimate time, rats exposed to lead as juveniles were evaluated on a minimum response duration task (Cory-Slechta et al. 1981).
When raw data and reads can be more directly evaluated in integrated viewers, and the distinction between signal and noise (as a result of either a chemistry artifact in the raw data or alignment artifacts at the analysis/interpretation stage) can be more easily assessed, it is likely that this threshold value can be reduced.
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Of course, if observed activity data were available in replicate, one could more directly evaluate whether a landscape or cliff were different from noise.
In order to more directly evaluate whether CO induced barrier permeability, we assessed the movement of a fluorescence-labelled albumin across the endothelial and epithelial barriers.
Further studies are warranted to analyze the potential of hepcidin as a biliary stress marker as well as to more directly evaluate its contribution to the defence against biliary infections.
We first used mice genetically deficient in NADPH oxidase (Morgenstern et al., 1997) to more directly evaluate the importance of this pathway in vivo.
Because ours was a study of incidence rather than mortality, we could more directly evaluate potential risk relationships without potential confounding by factors related to prognosis.
Hopefully, you can perform these additional experiments to more directly evaluate the kinase-like functions of CylM and the potential role of the ADP product in the second phosphate elimination step.
To more directly evaluate the contribution of each PcNLP gene to P. capsici toxicity, we employed qRT-PCR to evaluate transient expression of each silenced gene in putative transformant lines compared with SD33 and CK, using RNAs extracted from growing mycelia.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com