Frontal cortex exhibited more diglycosylated and nonglycosylated forms than the cerebellum.
In the frontal cortex (Fig 3B, 3C), thalamus (Fig S2B, S2C) and cerebellum (Fig S3C) from VV patients, diglycosylated forms were significantly more abundant compared to MV patients and MM patients.
Here, we present data on the thermodynamic stability and conformational dynamics of the mono- and diglycosylated forms.
Higher amounts of diglycosylated forms were detected in the thalamus while an increased accumulation of monoglycosylated forms occurred in the cerebellum (Fig 2D).
It is noteworthy that besides sCJD, vCJD and FFI are associated with a high content of diglycosylated forms and are characterized by a "targeting" of the thalamus.
As a matter of fact, we found in sCJD cases that the thalamus favored the production of type 2 PrPres presenting a high content in diglycosylated forms.
Most of the GPLs produced by M. smegmatis mc155 were diglycosylated forms, confirming earlier reports [ 15].
The western blots probed with anti-prion antibody, SAF-83, showed three characteristic anti-prion antibody reactive bands corresponding to the unglycosylated, monoglycosylated, and diglycosylated forms of PrPSc.
Valine homozygosity favoured the diglycosylated form, methionine homozygosity favoured the monoglycosylated form, and heterozygosity favoured the nonglycosylated form.
