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For a few of the more difficult sequences, we used 5' or 3' RACE or RT-PCR to verify/complete the sequence.
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Most likely due to their great sequence diversity rendering homology searches across species more difficult, the sequencing of the human genome had revealed many new GPCRs.
Grouping different sequences with similar structural properties for example, will always be more difficult for sequence-based methods, as was shown for predicting prokaryotic TF binding sites.
DNA extracted from paraffin-embedded tissues was also more difficult to sequence than DNA from frozen tissue [47], [48].
GC rich regions are known to be more difficult to sequence [ 21].
These results indicated that A-subgenome BACs possessed regions more difficult to sequence than those from the D-subgenome.
This finding suggests that sw1, sw2 and lw conopsins are located in GC-rich regions (such as the microchromosomes), which are known to be more difficult to sequence.
The D-subgenome had an average of 1.48 gaps/BAC, demonstrating that BACs from the A-subgenome are more difficult for sequence assembly than those from the D-subgenome.
Like SSF, the length of the sequence became increasingly more difficult (from a sequence of 2 blocks to a maximum sequence of 8 blocks) until they obtained the required number of errors for discontinuation.
This task is more difficult for metagenomic sequences because the origin of the sequence is almost always unknown (since the metagenome is a mixture of sequences from different species) and, consequently, the construction of a reference tree is not straightforward.
The sequences became increasingly more difficult (from a sequence of two digits to a maximum of nine digits) until the children obtained the required number of errors for discontinuation.
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