The in vivo and in vitro association of S100A16 with a more differentiated phenotype led us to investigate whether S100A16 can induce expression of differentiation-related markers in OSCC-derived cells.
NKG2A+CD57+CD56dim cells may be in an intermediate differentiation phase, retaining responses to cytokine stimulation and in transition to a more differentiated phenotype [24].
Cells exhibited a more differentiated phenotype on the PEGDMA DEGDMA copolymers compared to the 2HEMA PEGDMA copolymers.
On the PEGDMA DEGDMA system, cells exhibited a more differentiated phenotype on the stiffest surface indicated by elevated osteocalcin compared with TCPS.
Previously, we showed that osteoblasts exhibit a more differentiated phenotype when grown on machined or grit-blasted titanium aluminum vanadium (Ti6Al4V) alloys with micron-scale roughened surfaces than when grown on smoother Ti6Al4V surfaces or on tissue culture polystyrene (TCPS).
In addition, extremely low-frequency electromagnetic fields treatment induced a more differentiated phenotype as well as an increased expression in peroxisome proliferators-activated receptor isotype β, a class of transcription factors related to neuronal differentiation and cellular stress response.
In addition, chondrocytes expanded in the serum-free medium with FGF2 expressed a more differentiated phenotype at the end of monolayer culture, as indicated by higher gene expression ratios of collagen type II to collagen type I and aggrecan to versican.
However, the RNA-T cells were more heterogeneous; they had a more differentiated phenotype (50.8% CD45RO/CCR7 double-positive), and 52.5% of the cells expressed CD62L and CD28 (Fig. 3B).
Consistent with their more differentiated phenotype, CD4- NKT cells were predominantly resistant to functional reprogramming and displayed higher cytotoxic function.
As CMV infection is typically well controlled in adults, this suggests a more differentiated phenotype may be beneficial [12].
Together, these indicate that the overall phenotypic change in Dicer-null NS cells does not correspond to a simple shift towards or away from a more differentiated phenotype.
