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Conversely, cells on 2HEMA PEGDMA copolymers became more differentiated on the less stiff 2HEMA surface.
Alizarin red quantification indicated that both hMSCs and osteoblastic cells were more differentiated on TCPS than on Ti and glass.
In neuroblastoma, favourable tumour subtypes have been described to be more differentiated on the molecular level (Ohira et al, 2003; Nakagawara, 2004; Fischer et al, 2006).
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This data points to the conclusion that in the poorly differentiated HCC cell line, the efflux is mainly depend on the ABCG2 activity while in the more differentiated ones, drug export is also accounted by the activity of ABCB1.
Male and female cells responded similarly to Ti microstructure with respect to cell number and levels of osteocalcin, transforming growth factor-β1, osteoprotegerin and prostaglandin E2 in their conditioned media, exhibiting a more differentiated phenotype on SLA than on PT or TCPS.
On the PEGDMA DEGDMA system, cells exhibited a more differentiated phenotype on the stiffest surface indicated by elevated osteocalcin compared with TCPS.
It therefore is of crucial relevance to include on the one hand more differentiated phenotypic measures, on the other hand functional aspects on the cellular and molecular level as well as biological network analysis approaches to focus on the most relevant findings.
"His intention is to raise awareness for the necessity of a much broader, deeper and more differentiated debate on the future of the human being, and of being human, in an age where the human body is beginning to melt with technology," Benedikter adds.
Cells exhibited a more differentiated phenotype on the PEGDMA DEGDMA copolymers compared to the 2HEMA PEGDMA copolymers.
Moreover, instead of bold political statements, it demonstrates the relevance of a more differentiated perspective on the downsides and benefits of European free movement.
They introduced the concept of the mixed embeddedness, which tries to overcome the dualism of structure and culture (individual) and leads to a more differentiated perspective on migrant entrepreneurship.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com