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Two or more different sequences from the same strain were kept.
The mapped reads were considered concatenated sequences that encode histone proteins when two or more different sequences were mapped within 90 nt of each other.
When DNA mixtures containing two or more different sequences are used as templates for PCR, heteroduplexes are formed automatically during cycles of denaturing and re-annealing.
FSCI takes a range of values between 0 and 1: the higher the value - the more different sequences have MFE related signals inside the corresponding neighborhood (higher signal conservation), the lower - the less sequences have MFE related signals in common (lower signal conservation).
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The observation that there are considerably more different sequence signatures present in the earliest DPAs of ovule development (0 DPA to 2 DPA) than in later periods may indicate that there is a shift in the regulatory landscape after 2DPA with fewer small RNAs present but in higher numbers later on.
Chimeric sequences, which include two or more different mRNA sequences in an EST sequence, were then surveyed.
Given the high-confidence sequencing coverage and verification of this bi-allelic SNP from independently-prepared CPC98 DNA extracts using two sequencing technologies, we believe that this finding most probably represents mtDNA sequence heteroplasmy namely the existence of two or more different mtDNA sequences within the same cell/tissue/individual (Figure 2).
If the identified GCNVs involved gene duplications or multiplications, three or more different allelic sequences should be observed within the gene in each individual of each group, because three or more different allelic sequences cannot originate from a diploid genome.
Thus, we examined whether three or more different allelic sequences were observed in the identified GCNVs.
Over 77% of the proteins included are identified from two or more different peptide sequences as shown in Fig. 3, where identification is defined by both peptide detection and a protein sequence match.
Because the generation of heterozygous signals is due mainly to the binding of the SNP probe to two or more different genomic sequences (i.e., non-specific binding), the detected signal may not represent the genotype corresponding to the SNP probe itself.
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