More detailed transcriptome-specific information for the individual CC founder strains may also provide additional insight into previously reported strain-specific phenotypes [ 32- 34], improved imputed transcriptomes/genomes for individual strains and resultant crosses [ 28], and guidance for strain specific therapeutics models [ 35, 36].
Microarray technology provides a traditional profiling method to measure thousands of known genes simultaneously, but it has recently been replaced by RNA-seq technology that has proven to give more detailed insights into transcriptome.
We have included a more detailed description of the transcriptomes in the Results section: "Transcriptome sequencing revealed that all predicted toxin-related genes of the SOX symbionts in B. azoricus and B. sp gills were expressed.
Due to NGS providing a more detailed observation at the transcriptome, scientists and biologists have been eager to apply NGS for gene expression profiling.
In order to provide more detailed information about its transcriptome we subjected normalized larval cDNA to NextGen pyrosequence (Roche 454 FLX) and Sanger analysis.
More detailed analysis of the transcriptome with the motifs of proteins known to be associated with cytokine signaling was useful for the elucidation of other important genes from F. hepatica.
Microarrays are cheap and reliable tools for monitoring transcript changes, and although RNA-seq methods may allow a more detailed view inside the transcriptome, the lower costs, the high reproducibility and the established analysis systems advantages microarrays for routine applications.
Lower requirements for the amount of starting RNA have enabled gene expression profiling to be combined with cell sorting methods or laser capture microdissection, while increases in the number of features represented on the arrays have resulted in progressively more detailed coverage of the transcriptome.
The complete transcriptomes appeared to show more general functions, such as metabolism, transport or transcription, whereas the differentially expressed transcriptomes presented more detailed terms and functions, such as defense or regulation of specific signaling processes.
To find candidate genes that catalyze the last step(s) to artemisinin, a more detailed analysis was needed of the transcriptome of glandular trichomes.
