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After the CMIP5 exercise, needs were recognized for more detailed protocols to handle anthropogenic land use and land-use change in land surface models.
More detailed protocols can be provided upon request.
More detailed protocols for tissue preparation and recording techniques are available in previous papers [8], [12], [21].
All primers are listed in Table S4; more detailed protocols and PCR conditions are available upon request.
For more detailed protocols, see online supplementary methods.
Maps of the DNA constructs used and more detailed protocols for protein expression are available on request.
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A more detailed protocol can be found in Supplementary information, Data S1.
Interested readers are encouraged to refer to [8] for more detailed protocol specifications and technical discussions.
For a more detailed protocol including buffers, see Millipore's online ChIP protocol.
However a more detailed protocol with more markers for each studied vertebral segment would allow a more precise three-dimensional monitoring of spinal kinematics [34].
No nebulization step was required because the input amplicons already ranged in size from 300 600 bp, and a more detailed protocol can be found in Supplemental File S1.
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