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A more detailed protocol can be found in Supplementary information, Data S1.
Interested readers are encouraged to refer to [8] for more detailed protocol specifications and technical discussions.
For a more detailed protocol including buffers, see Millipore's online ChIP protocol.
However a more detailed protocol with more markers for each studied vertebral segment would allow a more precise three-dimensional monitoring of spinal kinematics [34].
The sensitivity of a standard postmortem procedure (including mycobacterial culture) for detecting M. bovis relative to a more detailed protocol was estimated to be around 54% in a study of 205 badgers [18].
No nebulization step was required because the input amplicons already ranged in size from 300 600 bp, and a more detailed protocol can be found in Supplemental File S1.
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After the CMIP5 exercise, needs were recognized for more detailed protocols to handle anthropogenic land use and land-use change in land surface models.
More detailed protocols can be provided upon request.
All primers are listed in Table S4; more detailed protocols and PCR conditions are available upon request.
More detailed protocols for tissue preparation and recording techniques are available in previous papers [8], [12], [21].
For more detailed protocols, see online supplementary methods.
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