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More detailed localization studies on the cytoplasmic NEK2A concurred to show that it is a core component of the centrosome [ 17].
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Our in vitro study provided a more detailed subcellular localization of these proteins, indicating that they are expressed along the lateral surface of the membrane and at intercellular junctions in the confluent state.
The 27 B. pahangi and 39 B. malayi predicted secretory proteins coded for by preferentially expressed transcripts were further analyzed by PSORTII [ 24] and ProtComp http://www.softberry.com; two protein algorithm that give more detailed protein localization predictions.
To analyze more detailed subcellular localization of misfolded SOD1 in spinal motor neurons of SOD1 G93A -Tg mice, we divided the spinal cord into four subsegments by laser-assisted microdissection (LMD) (summarized in Fig. 1A) and used the lysate from each segment to detect SOD1 by immunoblot analysis.
For the few constructs generated in our laboratory, a more detailed analysis of their localization was performed using co-localization experiments with plasma membrane or nucleus markers (not shown).
To get a more detailed picture of the localization of flotillins on endosomal structures, we took advantage of a HeLa cell line expressing a constitutively active Rab5 mutant (Q79L) in an inducible manner.
To obtain more detailed information about aPKC localization, we captured confocal z-stack images in late-neurula and early-tailbud stage embryos and reconstructed sections perpendicular to the AP axis.
We then analyzed a smaller cohort of melanoma patients ("English" cohort) for a more detailed analysis of CITED1 localization.
The availability of a sensitive and highly specific antibody against CHMP4B allowed us to perform a more detailed study of the localization of this protein by confocal microscopy.
A more detailed analysis of the localization of the incorporated Fluo-3 AM in the pistil at stages 4 and 5, which are highly significant for sexual plant reproduction events in flowering plants, was also performed.
To obtain more detailed information on subcellular localization of PANS1 in meiocytes, we generated a FLAG-tagged PANS1 line in a pans1 mutant background in which the pans1 mutant was complemented by the FLAG-tagged allele.
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