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A more densely staining region of Brdt protein appeared to separate sirtuin 1 (Sirt1) protein from contact with the chromocenter.
Most of the repeated sequences are packaged into heterochromatin – condensed and mostly transcriptionally silent chromatin identified cytologically as being more refractile and more densely staining [ 10].
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On the basis of qualitative comparisons, it appeared, in general, that there were more densely stained AC3-positive profiles in the P2+P7 PCP-treated brains compared to that observed in the P7 alone PCP group.
In this bud, the cytoplasm became more densely stained than before self-pruning.
Moreover, neurons in adjacent cortex also exhibit intranuclear but more cytoplasmic polyQ deposits; therefore, they are more densely stained in comparison with striatal neurons, particularly in tgHD rats– 8(g)).
Based on cytogenetic observations, there are two main classifications of chromatin: lightly staining euchromatin which undergoes cell-cycle-dependent stages of condensation and de-condensation, and the more densely stained heterochromatin that remains compact throughout most of the cell cycle [ 35].
These included early changes such as scattered angular, atrophic fibres which are densely stained with NADH-TR as well as more chronic changes with small and large grouped atrophy and fibre type grouping.
STED offers the advantage of allowing access to the centre of the cell, with reduced acquisition time, and is less prone to artefacts of densely stained samples; however, the experimental setup is more complex.
The antipodal cells are located at the chalazal end and densely stained.
When compared to the original leaf cells, the meristematic cells showed smaller size and obviously densely stained (Fig. 3a).
The apical membrane of the choroid plexus epithelium was densely stained, implying a role for CHIP in the secretion of cerebrospinal fluid.
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