Sentence examples for more deep sequencing from inspiring English sources

More deep sequencing will be necessary to correlate these short RNAs with the whole genome tiling array studies [ 67], which identify the signal deriving from short RNAs overlapping TSS of mRNAs We have discovered multiple classes of small RNAs derived from many different sources, in addition to six novel miRNAs.

The different single nucleotide profiles and poly(A) signals among different genic regions in C. reinhardtii indicate that polyadenylation mechanism may be diversified in eukaryotes, and more deep-sequencing data (beyond 3′-UTRs) are needed to give further analysis in other genic regions beyond 3′-UTRs.

As a result, more sensitive deep sequencing assays are needed to identify lower frequency off-target mutations (Cho et al., 2014; Fu et al., 2014).

The diagnosis of reinfection has traditionally been based on direct Sanger sequencing of samples pre- and posttreatment, but not on more sensitive deep sequencing techniques.

The development of functional genomics tools, such as microarray and more recently deep sequencing technology, as well as proteomics, has revealed strategies to achieve clinical goals [ 89].

More recently, deep sequencing technologies allowed the identification of new types of small RNAs derived from the processing of already known sncRNAs including tRNA (Rutjes et al., 1999; Rother & Meister, 2011; Sobala & Hutvagner, 2011).

More recently, using deep sequencing technology, two groups separately found that miR-K12-7 cactuallylly gives rise to two mature miRNAs, miR-K12-7-5p and min-K12-7-3p, in different KSHV-positive cell lines [12], [13].

More recently, large-scale detection of ER binding sites by chromatin immunoprecipitation (ChIP), initially combined with tiling arrays and more recently with deep sequencing, have established genome-wide maps (reviewed in [ 58]).

More recently, newer deep sequencing technologies have been used to profile microRNAs in Arabidopsis DICER and RDR2 mutants [ 4, 5], and others have applied this technology to various samples including human and chimpanzee brain [ 6] and Chlamydomonas reinhardtii [ 7].

These genotype data can be generated by PCR amplification using primer pairs flanking the markers followed by capillary electrophoresis or, more recently, from deep sequencing of these ampliconic products [ 14, 15].

These data suggest that ultra-deep sequencing using short read technology clearly defines more transcribed regions than deep sequencing with 454 technology and may also define many more putative alternate splicing events.