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Exact(8)
The extent of boundary modification was a function of the number of applied loading cycles and the testing temperature, with more cycles at higher temperatures leading to more special grain boundaries.
Primer annealing followed a touchdown strategy, starting with 55°C and decreasing 1°C every two cycles during the first 20 cycles, followed by 15 more cycles at 45°C, always for 30 sec.
PCR was achieved with 35 more cycles at 52°C.
The reaction proceeded for 15 more cycles at an annealing temperature of 55 °C.
This underestimation of fecundability, due to inclusion of couples with one or more cycles at risk before follow-up, was demonstrated by de Mouzon et al. (1988).
All patients at the first dose level (C 1100, D 50, E 110 x 3) completed three or more cycles at the dose and schedule planned by the protocol and no 'dose-limiting toxicity' (DLT) was seen.
Similar(52)
However after two or more cycles and at 1 year, AMH was undetectable or close to the limit of detection in all women.
PCR cycling parameters were as follows: (1) 2 minutes at 95°C, (2) 30 seconds at 95°C, (3) 30 seconds at annealing temperature gradient, (4) 1 minute at 72°C, (5) repeat steps 2 4 for 34 more cycles, (6) 5 minutes at 72°C.
The PCR cycling parameters were: (1) 30 seconds at 98°C, (2) 7 seconds at 98°C, (3) 20 seconds at appropriate annealing temperature, (4) appropriate extension time at 72°C, (5) repeat steps 2 4 for 29 more cycles, (6) 7 minutes at 72°C.
Such steps should be especially considered when a patient has demonstrated tolerance and stable renal function after one or more cycles of carfilzomib at 27 mg/m and also, obviously, in the setting of CHF.
The dominant sequence was further enriched with more cycles of SELEX; at the seventh round, 12 of 16 clones sequenced had the identical sequence of the dominant picked up at the third round, suggesting that this sequence exhibits the highest affinity for PhoP among members of the selected DNA pool.
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