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The DnaJb subfamily members tend to show more consistent expression across brain regions, with several being consistently in the lower 20th percentile.
More importantly, the more consistent expression signature of 4 sex genes in the post-globin reduction group with respect to the pre-globin reduction group indicates that class prediction was markedly improved with globin reduction.
However, analysis of CCR5 expression revealed higher levels of CCR5 on CD3+DP (HIV+ 2.2%, HIV- 0.7%) and SPCD8+ thymocytes (HIV+ 4.71%, HIV-1.7%) in HIV-infected thymus compared to uninfected controls, suggesting a possible role by higher immune activation status in the induction of a more consistent expression of CCR5 on thymocytes.
Moreover, the spike-in-based normalization method provided more consistent expression patterns in multiple samples with the qRT-PCR measurements (Fig. 2e).
We have compiled a set of high quality HKGs that should provide higher and more consistent expression when used as references in laboratory experiments than currently used HKGs.
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Furthermore, regardless of the expression level, RNA-Seq appears to produce more consistent gene expression patterns between brains than microarray.
Based on the more consistent GFP expression patterns obtained using lima bean cotyledons, transient expression analysis of all 20 different cloned soybean promoters was conducted using lima bean cotyledons as the target tissue.
The data demonstrate that intradermal DNA delivery in combination with electroporation results in cell transfection throughout all layers of the skin and yields faster, higher and more consistent gene expression, compared to intradermal gene delivery alone.
In contrast, the inner epithelia had more consistent p63 expression and were less prone to disintegration.
Alternative IRES elements like the 9-nucleotide IRES element from the murine Gtx homeodomain gene [ 35], or eliminating the IRES entirely, may allow for more consistent tTA expression.
On the other hand, transformation using Agrobacterium typically results in lower copy number gene introductions, which has been reported to give more consistent transgene expression [ 63, 64].
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