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In general, information content tends to be larger at more conserved sites.
Fewer substitutions are expected to occur in more conserved sites.
Only a third of the human/mouse orthologous gene pairs contains one or more conserved sites by this definition.
Within the basic region, fungal sequences contained more conserved sites and lower entropy values in general [ 20].
The distribution of variable sites and conserved sites suggests that Domain II has relatively more conserved sites and Domain I has more variable sites compared to other domains.
Splitting noncoding sites into ten groups based on GERP score, we again observe a gradient in the depth of depression of average MAF in the surrounding sequences, with more conserved sites causing the deepest depression (Additional file 1: Figure S21).
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For example, the drop in performance observed between TargetScan.All and TargetScan.Cons illustrates the effect of limiting analysis to the more highly conserved sites.
Poly(A) sites were classified as major/minor or narrow/broad as described above, and their spatial organization was observed in genes containing two or more tandem conserved sites.
These analyses revealed that when fast evolving sites are excluded from the data and more relatively conserved sites are present, the hypothesis of relationships between Rab proteins agreeable with species phylogeny can not be rejected or even is favoured.
For example, biodiversity conservation may be much more effective if conserved sites are connected.
When applied to human chromosome 22, these filters resulted in the identification of ~41 600 mutated non-coding sites, and roughly one hundred times more non-coding conserved sites.
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