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T-cells, which mediate cellular immune responses, can target more conserved peptides from internal proteins and have the potential to provide wider protection.
Included three or more conserved peptides from a subfamily.
Lower entropy scores correspond to more conserved peptides (Frahm et al., 2004).
As previously described [ 25] a hit was considered significant if one of the open reading frames in the sequence: Included three or more conserved peptides from a subfamily.
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Thus, the most variable regions of TR (CDR3) are positioned in the center of binding interface where they contact the peptide, whereas the more conserved regions of TR (CDR1 and CDR2) and the tops of MHC helices engage in contacts that surround the central CDR3-peptide region like a "gasket" [4].
Cyclic peptides may therefore carry more conserved epitopes, T-cell epitopes, and possibly conformational epitopes that are shared by Opa proteins with a less related primary sequence.
Overall, reactive peptides were more likely to correspond to more conserved portions of the HIV-1-B Nef protein - the 350 peptides that elicited ELISpot responses corresponded to viral regions of significantly lower Shannon entropy than the HIV-1 regions encompassing the 594 peptides that were not recognized (p<0.0001).
Influenza-specific T-cell immunity is often directed against peptide components derived from the more conserved internal viral gene products, such as nucleoprotein, matrix protein 1 or polymerase subunits.
Comparison of the conserved peptides between subfamilies showed that the AA9exp subfamilies shared from 0 to 10 conserved peptides.
Mature miRNAs from older and more conserved miRNAs contain less toxic seeds.
All patients (100%) had one or more conserved AF rotors between procedures with surrounding disorganization.
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